Auxo-Agro™ Methionine Auxotrophic Agrobacterium Competent Cells

About

Are antibiotics hurting your plant transformants? Agrobacterium removal is needed following a successful plant transformation. Traditionally, antibiotics are used for this purpose, but higher concentrations of antibiotics can have an adverse effect on plant tissue. GoldBio EHA105∆Met 1 and LBA4404∆Met 1 Agrobacterium tumefaciens competent cells minimize the use of antibiotics to prevent overgrowth of the plant tissue and can help streamline your plant transformation procedures. Benefits: Reduces the need for antibiotics during transformation . Increases plant transformation efficiency. Agrobacterium can be completely killed by removing methionine from the culture media combined with a low concentration of antibiotic. Knocking out genes to cause auxotrophy DOES NOT affect transformation capacity. 1 Prías-Blanco M, Chappell TM, Freed EF, Illa-Berenguer E, Eckert CA, Parrott WA. An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation. Transgenic Res. 202231: 661-676. doi: 10.1007/s11248-022-00328-4. PMID: 36239844. Important note from this paper : “Switchgrass transformation was chosen to validate these strains by evaluating their performance in a difficult transformation system."