pSuperAgro® Plasmids

About

Check out these new helper plasmids! pSuperAgro ® plasmids are designed to include either AcdS or GabT activity or both together, driven by a single lac promoter. The purchase of this product includes a 1-year subscription to use pSuperAgro ® in your research. License Agreement These products are sold under license by GoldBio and require a signed agreement before fulfillment. Benefits pSuperAgro ® 1 Ethylene evolution suppression Higher callus induction frequency Reduced callus browning pSuperAgro ® 3 Reduced GABA content Induces higher shoot regeneration Increased transient and stable transformation efficiencies pSuperAgro ® 4 Ethylene evolution suppression Reduced GABA content Higher callus induction frequency Reduced callus browning Induces higher shoot regeneration Increased transient and stable transformation efficiencies pSuperAgro ® Plasmids Check out these new helper plasmids! pSuperAgro ® plasmids are designed to include either AcdS or GabT activity or both together, driven by a single lac promoter. GabT GabT activity, driven by the lac promoter, degrades gamma-aminobutyric acid (GABA) during co-cultivation. The reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. GabT activity decreases GABA content locally in plant cells and induces higher shoot regeneration, decreasing the number of cotyledons used for transformation and reducing the time and labor required for transformation. This plasmid has been shown to be efficacious in both Hardy Pampas Grass ( E. Ravennae ) and Tomato plants ( S. Lycopersicum ). AcdS 1-aminocyclopropane-1-carboxylate (ACC) deaminase (AcdS) activity is driven by the lac promoter which suppresses ethylene evolution during co-cultivation and significantly increases T-DNA transfer. AcdS activity is beneficial for higher callus induction frequency and suppresses callus browning phenomena. This plasmid has been shown to be efficacious in b